Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

Abstract The goal of the present study was to evaluate effects enzymes (cellulase combined with galactosidase) and their combination Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated or without cellulase + ɑ-galactosidase (CEGA), LP (CELP), (GALP). After 56 days ensiling, all exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N increased lactic acid levels compared control silage ( P < 0.05). Enzymatic treatment nutrient value increasing crude protein decreasing neutral detergent fibre (NDF) Silage significantly altered community, determined PCoA Lactic bacteria (LAB) dominated community after ensiling. dominant changed from Garciella , Enterococcus Pediococcus CEGA CELP GALP silages. Collectively, these results suggest that both alone inoculants greatly abundance LAB, observed (CEGA) a (CELP GALP).